Yeast Culture

Coordinator:
Nathalie Tessier
(514) 252-3400 ext. 3334
ntessier.hmr@ssss.gouv.qc.ca

The Yeast Culture Program aims to:

  • Conduct studies to reveal new genetic and biochemical factors with regard to cell sensitivity and resistance to different antineoplasic drugs;
  • Identify new molecular targets and their particular molecules in basic and medical research projects.

Services

  • Yeast haploid mutant tiling intended for studies on antineoplasic drug tolerance such as bleomycin.
  • Dissection of yeast tetrads with deletion combinations of genes that are specific to antineoplasic drug resistance.
  • Analysis on intracellular distribution of antineoplasic drugs previously marked in tissue biopsies.
  • Validation of the different multiple gene expression profiles (about 30) in a single sample.
  • Purification of proteins and of selected protein complexes.

Equipment

  • Automated pipetting platform
  • Yeast micromanipulator
  • Zeiss Axio Observer microscope with analysis software
  • Multiple gene expression analysis device (GeXP of Beckman-Coulter)
  • FPLC chromatography system

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