Flow Cytometry
Coordinator: 
Martine Dupuis
(514) 252-3521
mdupuis.hmr@ssss.gouv.qc.ca
Flow cytometry is a technique that consists of streaming particles or cells before a laser beam. Fluorescent or scattered light emitted back serves to measure and analyze their physical characteristics; to identify subsets of cells; and to physically separate them (cell sorting).
More than 15 research units, particularly the Immunology-Oncology Research Axis, use flow cytometry devices.
Services
- Immunophenotyping
- Cell cycle
- Apoptosis analysis
- Intracellular calcium measuring
- GFP/YFP transfected cell sorting
- Slide sorting (for clonal expansion), and in tubes of various sizes
The Cytometry Program also provides users with training that is tailored to their particular needs, and consulting sessions when developing experimental protocols (choice of fluorochromes, control, types of markings, etc.).
Equipment
- Four BD Biosciences analyzers:
- FACScan that can analyze 3 colors;
- FACScalibur that can analyze 4 colors;
- FACSCANTO that can analyze 6 colors.
- BD LSR II that can analyze twelve parameters (10 colors).
- Cell sorting:
- FACS Aria III equipped with 4 excitation sources for a possibility of 13 colors
- AutoMACS Miltenyi Biotech
- Softwares avalaible: BD Cellquest Pro, BD DiVa, BD FCAP Array, Modfit (Verity Software) and FlowJo (Treestar)