Flow Cytometry
Coordinator: 
Martine Dupuis
(514) 252-3521
mdupuis.hmr@ssss.gouv.qc.ca
Flow cytometry is a technique that consists of streaming particles or cells before a laser beam. Fluorescent or scattered light emitted back serves to measure and analyze their physical characteristics; to identify subsets of cells; and to physically separate them (cell sorting).
More than 15 research units, particularly the Immunology-Oncology Research Axis, use flow cytometry devices.
Services
- Immunophenotyping
- Cell cycle
- Apoptosis analysis
- Intracellular calcium measuring
GFP/YFP transfected cell sorting - Slide sorting (for clonal expansion), and in tubes of various sizes
The Cytometry Program also provides users with training that is tailored to their particular needs, and consulting sessions when developing experimental protocols (choice of fluorochromes, control, types of markings, etc.).
Equipment
- FACS Vantage SE cell sorter of BD Biosciences with DiVa option and turbo sort that can simultaneously sort 4 subsets.
- BD LSR II that can analyze ten parameters, hence ten colors.
- Three BD analyzers of Biosciences:
- FACScan that can analyze 3 colors;
- FACScalibur that can analyze 4 colors;
- FACSsort that can analyze 4 colors.
- Four analysis stations equipped with BD Cellquest Pro, BD DiVa, Modfit (Verity Software) and FlowJo (Treestar) software.