Bioimaging


Coordinator :

Mikhail Sergeev, Ph.D.
514-252-3400 ext.: 5095
mikhail.sergeev@umontreal.ca

The Bioimaging core facility provides CRHMR research investigators with cutting-edge optical microscopy equipment, training and support in developing future microscopy experiments.

equipment

Optical Microscopy

STATION.1    Inverted confocal / multi-photon Olympus FV1000 LSM system

    • Olympus IX81 motorized inverted microscope
    • Olympus FluoView FV1000 scanhead with spectral detectors
    • Laser merge module with AOTF controlled lasers: 405nm, 458nm, 488nm, 515nm, 543nm & 635nm
    • SpectraPhysics MaiTai DeepSee Ti:Sapphire femtosecond pulsed laser
    • Two non-descanned multialkali PMT detectors
    • QImaging Retiga EX monochrome CCD camera
    • Prior Proscan III motorized translational stage
    • HBO100 light source
    • Vibration isolation optical table
    • Olympus Fluoview and cellSens software

       

      STATION.13    Upright multi-photon Zeiss LSM880 system

       

        • Upright multi-photon Zeiss LSM880 system
        • Zeiss AxioExaminer Z1 motorized upright microscope
        • Zeiss LSM880 scanhead with spectral detectors
        • SpectraPhysics MaiTai DeepSee Ti:Sapphire femtosecond pulsed laser
        • Ultra deep imaging with water dipping W Plan-Apo 20x/1.0 WD=1.8
        • Fast intensity control via AOM
        • Four non-descanned multialkali PMT detectors
        • Prior Proscan motorized translational stage
        • HXP120 metal-halide light source
        • Waner Instruments micro-incubation perfusion system
        • Vibration isolation optical table
        • Zeiss Zen Black and Zen Blue software


          STATION.2   Laser capture microdissection Zeiss system

           

            • Zeiss PALM microbeam with 350nm solid-state laser
            • Zeiss AxioObserver Z1 motorized inverted microscope
            • Zeiss Apotome for optical sectioning
            • X-Cite metal-halide light source
            • AxioCam MRm monochrome and AxioCam ICc1 color CCD cameras
            • motorized translational stage
            • Zeiss AxioVision and PalmROBO software


              STATION.3    DeltaVision Elite imaging system

               

                • Olympus IX71 inverted microscope with motorized fast filter wheels
                • UltimateFocus focal drift correction system
                • Motorized XYZ high precision stage
                • Incubation chamber for long term live cell imaging
                • LED light source
                • sCMOS monochrome camera
                • non-blind deconvolution for optical sectioning
                • GE SoftWoRx software


                  STATION.4    Live cell Zeiss imaging system

                   

                    • Zeiss AxioObserver Z1 motorized inverted microscope
                    • TokaiHit top stage incubation chamber for long term live cell imaging
                    • ThorLabs MLS203 high precision translational stage
                    • Photometrics CoolSnap HQ cooled monochrome CCD camera
                    • HBO100 light source
                    • MetaMorph image acquisition software


                      STATION.5    Upright fully motorized widefield Zeiss imaging system

                       

                        • Zeiss AxioImager Z2 upright motorized microscope
                        • AxioCam MRm monochrome and AxioCam MRc color CCD cameras
                        • Motorized high precision translational stage
                        • HBO50 light source
                        • Zeiss AxioVision software


                          STATION.6    Ratiometric Zeiss widefield imaging system

                           

                            • Zeiss AxioObserver A1 inverted microscope
                            • Fast switching Xenon Sutter DG5 light source for ratiometric imaging
                            • Sutter Lambda 10-3 with fast switching emission filter wheel
                            • QImaging Retiga EX monochrome CCD camera
                            • EMPIX Northern Eclipse software

                              STATION.7    Upright widefield Nikon imaging system

                               

                                • Nikon Eclipse E800 upright microscope
                                • Photometrics CoolSnap CF monochrome CCD camera
                                • Motorized high precision translational stage
                                • HBO100 light source
                                • MicroManager software

                                  STATION.8    Inverted widefield Olympus imaging system

                                    • Olympus IX73 inverted microscope
                                    • Olympus U-HGLGPS 130W light source
                                    • Olympus XM10 monochrome and SC30 color CCD cameras
                                    • Olympus cellSens software


                                      STATION.9   Upright widefield Olympus imaging system

                                        • Olympus BX53 inverted microscope
                                        • Olympus U-HGLGPS 130W light source
                                        • Olympus XM10 monochrome CCD camera
                                        • Olympus cellSens software


                                          STATION.10   Upright widefield Leica imaging system

                                            • Leica DMRE upright microscope
                                            • HBO100 light source
                                            • QImaging QCam monochrome CCD camera
                                            • Micromanager software


                                              STATION.11    Inverted widefield Leica imaging system – Biosafety level 2

                                                • Leica DM IRB inverted microscope
                                                • HBO100 light source
                                                • Leica DFC425c color CCD camera
                                                • Leica LAS software


                                                  STATION.12    Inverted widefield Olympus imaging system – Biosafety level 2

                                                   

                                                    • Olympus IX73 inverted microscope
                                                    • Olympus U-HGLGPS 130W light source
                                                    • Olympus XM10 monochrome and SC30 color CCD cameras
                                                    • Olympus cellSens software


                                                      In Vivo Functional vision Exploration

                                                      The platform is also equipped with the Heidelberg Laser Scanning Ophthalmoscope model HRA2 for retinal imaging applications on live animals. This instrument allows a functional exploration of the vision


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