Éric Milot

Office Phone: 514-252-3551
Fax: 514-252-3430
Lab Phone: 514-252-3400 # 3348/3330
e.milot.1@umontreal.ca

Research Axis  Immunology-Oncology
Research Unit  Malignant Hematopoiesis and Epigenetic Regulation of Gene Expression

TITLE

  • Associate Professor

EDUCATION

  • B.Sc., Biology, University of Sherbrooke (1986-1989).
  • Ph.D., Molecular Biology, University of Montreal (1989-1994).
  • Postdoctorate, Cellular Biology and Genetics, EUR, Netherlands (1995-2000). 

RESEARCH INTERESTS

We are studying the epigenetic regulation of gene expression during hematopoiesis.
More specifically, our studies focus on identifying factors and molecular mechanisms involved in epigenetic programming and regulation of gene expression. Genetically-modified mice, embryonic stem cells and human hematopoietic cells are used in our research.

Another aspect of our research consists of characterizing the factors that foster the development of leukemia. For these studies, we are examining how some of these factors influence regulatory pathways of cell proliferation, genomic stability and regulatory mechanisms of gene expression.

RESEARCH TEAM

Postdoctorate

  • Stefania Bottardi, M.D., Ph.D.
  • Nasser Fotouhi-Ardakani, Ph.D.

Ph.D. Student

  • Lionel Mavoungou
  • Farah Ahlam Zmiri 

Research Assistant

  • Vincent Bourgoin, M.Sc.

DISTINCTIONS AND ACHIEVEMENTS

  • Junior Investigator Grant 1, FRSQ (2000-2002).
  • New Investigator Award, CIHR (2002-2007).
  • Junior Investigator Award II, FRSQ (2007-2009).
  • Senior Investigator Award, FRSQ (2010 - 2013).

Selected papers

 

Ross J., Mavoungou L., Bresnick E. and Milot E. 2012. GATA-1 utilizes Ikaros and the Polycomb Repressive Complex 2 to suppress Hes1 and to promote erythropoiesis. Mol. Cell. Biol. 32(18):3624-38.

 

Bottardi S., Zmiri F.A., Bourgoin V., Ross J., Mavoungou L., Milot E. 2011. Ikaros interacts with P-TEFb and cooperates with GATA-1 to enhance transcription elongation. Nucleic Acids Res. 39(9):3505-19. 

 

Fotouhi-Ardakani N., El Kebir D., Pierre-Charles N., Wang L., Ahern S.P., Filep J.G. and Milot E. 2010. Role for Myeloid Nuclear Differentiation Antigen in the Regulation of Neutrophil Apoptosis during Sepsis. Am. J. Resp. Crit. Care Med.  182(3), 341-50.

  

Ross J., Bottardi S., Bourgoin V., Wollenschlaeger A., Drobetsky E., Trudel M. and Milot E. 2009. Differential requirement of a distal regulatory region for pre-initiation complex formation at globin gene promoters. Nucl. Acids Res. 37(16), 5295-5308

 

Bottardi S, Ross J, Bourgoin V., Fotouhi-Ardakani N., Affar E.B., Trudel M. and Milot E. 2009. Ikaros and GATA-1 combinatorial effect is required for silencing of human B-globin genes. Mol. Cell. Biol. 29(6), 1526-37  

 

Bottardi S., Ross J., Pierre-Charles N., Blank V. and Milot E. 2006. Lineage-specific activators affect ?-globin locus chromatin in multipotent hematopoietic progenitors EMBO J. 25(15), 3586-3595.

 

Bottardi S., Pierre-Charles N., Bourgoin V. and Milot E. 2005. Onset of mammalian position effect variegation can precede transcriptional activation during tissue differentiation. Hum. Mol. Genet. 14(4), 493-502.

 

Bottardi, S., Aumont, A., Grosveld, F. and Milot, E. 2003. Developmental-specific epigenetic control of human beta gene expression in hematopoietic progenitor cells. Blood, 104(12), 3989-3997.

 

McMorrow T., van den Wijngaarden A., Wollenschlaeger A., van de Corput M., Trimborn T., Fraser P., van Lohuizen M., Jenuwein T., Djabali M., Philipsen S., Grosveld F. and Milot E. 2000. Activation of the B globin locus by transcription factors and chromatin modifiers. EMBO J. 19, 4986-4996.

 

Whyatt D., Lindeboom F., Karis A., Ferreira R., Milot E., Hendriks R., de Bruijn M., Langeveld A., Gribnau J., Grosveld F. and Philipsen S. 2000. An intrinsic but cell nonautonomous defect in GATA-1 overexpressing mouse erythroid cells. Nature 406, 519-524.

 

Milot E., Strouboulis J., Trimborn T., Wijgerde M., de Boer E., Langeveld A., Kian T.U., Vergeer W., Yannoutsos N., Grosveld F. and Fraser P. 1996. Heterochromatin effects on the frequency and duration of LCR-mediated gene transcription. Cell 87, 105-114.


 

Complete list of Dr. Milot's publications (Pubmed)

ADDITIONAL INFORMATION

Research Grants

  • Leukemia Lymphoma Society Canada; Research project title: Role of the hematopoietic factor MNDA in genotoxic stress response.
  • Canadian Institutes of Health Research; Research project title: Mechanisms of Epigenetic Regulation and Hematopoietic Lineage Specification.

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