Internship in Molecular Mechanisms of Ubiquitination
Term: full time
Project description:
Ubiquitination, a post-translational modification of proteins, play a crucial role in a number of cell processes. Our laboratory is interested in understanding how ubiquitination regulates DNA transcription and repair. We are also trying to determine how ubiquitination defects contribute to tumor development (please visit our web page).
There are currently two main projects under way:
1) Molecular and functional characterization of ubiquitinate BAP1 and elucidation of its role in tumorigenesis.
2) Characterizing the roles of the tumor suppressor genes, BRCA1, in cell response to mutagenic agents.
Prerequisite:
- B.Sc. in Biology Sciences or equivalent;
- The student must be highly motivated.
Grant: the candidate will be paid according to the Centre’s salary range attributed to students.
Publications :
AA. Horwitz, EB. Affar, GF. Heine, Y Shi and J D. Parvin. A novel mechanism for transcriptional repression dependent on the BRCA1 E3 ubiquitin ligase. Proc. Natl. Acad. Sci. In press, (2007)
EB Affar, M Luke, F Gay, D Calvo, G Sui, RS. Weiss, E Li, and Y Shi. Targeted Ablation of Par-4 Reveals a Cell-Type Specific Susceptibility To Apoptosis-Inducing Agents. Cancer Research, 66(7):3456, (2006)
EB Affar, F Gay, Y Shi, H Liu, M Huarte, S Wu, C Tucker, E Li, and Y Shi. Essential dosage-dependent functions of the transcription factor Yin Yang 1 in late embryonic development and cell cycle progression. Molecular and Cellular Biology, 26(9):3565-3581, (2006)
G Sui, EB Affar, Y Shi, C Brignone, N R. Wall, P Yin, M Donohoe, D Calvo, M Luke, S R. Grossman, Y Shi. Yin Yang 1 is a negative regulator of p53. Cell, 117(7): 859, (2004)
Y Shi, J Sawada, G Sui, EB Affar, JR Whetstine, F Lan, H Ogawa, M Luke, Y Nakatani and Y Shi. Coordinated histone modifications mediated by a CtBP co-repressor complex. Nature, 422(6933):735, (2003)
G Sui, C Soohoo, EB Affar, F Gay, Y Shi, W Forrester, and Y Shi. A DNA vector-based RNAi technology to suppress gene expression in mammalian cells. Proc. Natl. Acad. Sci., 99:
Posting date: April 1, 2008
If you are interested, please send your cover letter, resume and university transcript by e-mail to:
Dr El Bachir Affar,Ph.D. , Research Investigator
Maisonneuve-Rosemont Hospital Research Centre
5415 Assomption Boulevard, Montreal, QC H1T 2M4
E-mail: aelbachir.hmr@ssss.gouv.qc.ca